NF-Y is a highly conserved heteromeric CCAAT-binding transcription factor involved in the function of several promoters. The NF-YA subunit contains a domain of high homology to yeast HAP2, which we show to be necessary and sufficient to mediate interactions with the NF-YB subunit and with DNA. Using protein affinity columns derivatized with amino acid substitution mutants, we further dissect this region into two functionally separable subdomains. The subunit association function resides in a 21-amino acid stretch, which is almost perfectly conserved among different species, while interaction with DNA resides in another short segment. We also show that DNA-binding mutants act as dominant repressors of NF-Y·DNA complex formation and of NF-Y- dependent transcription.
Dominant negative analogs of NF-YA / R. Mantovani, X.Y. Li, U. Pessara, R. Hooft van Huisjduijnen, C. Benoist, D. Mathis. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 269:32(1994 Aug 12), pp. 20340-20346.
Dominant negative analogs of NF-YA
R. MantovaniPrimo
;
1994
Abstract
NF-Y is a highly conserved heteromeric CCAAT-binding transcription factor involved in the function of several promoters. The NF-YA subunit contains a domain of high homology to yeast HAP2, which we show to be necessary and sufficient to mediate interactions with the NF-YB subunit and with DNA. Using protein affinity columns derivatized with amino acid substitution mutants, we further dissect this region into two functionally separable subdomains. The subunit association function resides in a 21-amino acid stretch, which is almost perfectly conserved among different species, while interaction with DNA resides in another short segment. We also show that DNA-binding mutants act as dominant repressors of NF-Y·DNA complex formation and of NF-Y- dependent transcription.File | Dimensione | Formato | |
---|---|---|---|
20340.full.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
6.39 MB
Formato
Adobe PDF
|
6.39 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.