A selective DNA pooling approach was applied to identify QTL for conjugated linoleic acid (CLA), vaccenic acid (VA) and D9-desaturase (D9D) milk content in Italian Brown Swiss dairy cattle. Milk samples from 60 animals with higher values (after correction for environmental factors) and 60 animals with lower values for each of these traits from each of five half-sib families were pooled separately. The pools were genotyped using the Illumina BovineSNP50 BeadChip. Sire allele frequencies were compared between high and low tails at the sire and marker level for SNPs for which the sires were heterozygous. An R procedure was implemented to perform data analysis in a selective DNA pooling design. A correction for multiple tests was applied using the proportion of false positives among all test results. BTA 19 showed the largest number of markers in association with CLA. Associations between SNPs and the VA and D9-desaturase traits were found on several chromosomes. A bioinformatics survey identified genes with an important role in pathways for milk fat and fatty acids metabolism within 1 Mb of SNP markers associated with fatty acids contents.

Quantitative trait loci mapping for conjugated linoleic acid, vaccenic acid and Δ9-desaturase in Italian Brown Swiss dairy cattle using selective DNA pooling / M.G. Strillacci, E. Frigo, F. Canavesi, Y. Ungar, F. Schiavini, L. Zaniboni, L. Reghenzani, M.C. Cozzi, A.B. Samorè, Y. Kashi, E. Shimoni, R. Tal Stein, M. Soller, E. Lipkin, A. Bagnato. - In: ANIMAL GENETICS. - ISSN 0268-9146. - 45:4(2014 Aug), pp. 485-499. [10.1111/age.12174]

Quantitative trait loci mapping for conjugated linoleic acid, vaccenic acid and Δ9-desaturase in Italian Brown Swiss dairy cattle using selective DNA pooling

M.G. Strillacci
Primo
;
E. Frigo
Secondo
;
F. Schiavini;L. Zaniboni;M.C. Cozzi;A.B. Samorè;A. Bagnato
Ultimo
2014-08

Abstract

A selective DNA pooling approach was applied to identify QTL for conjugated linoleic acid (CLA), vaccenic acid (VA) and D9-desaturase (D9D) milk content in Italian Brown Swiss dairy cattle. Milk samples from 60 animals with higher values (after correction for environmental factors) and 60 animals with lower values for each of these traits from each of five half-sib families were pooled separately. The pools were genotyped using the Illumina BovineSNP50 BeadChip. Sire allele frequencies were compared between high and low tails at the sire and marker level for SNPs for which the sires were heterozygous. An R procedure was implemented to perform data analysis in a selective DNA pooling design. A correction for multiple tests was applied using the proportion of false positives among all test results. BTA 19 showed the largest number of markers in association with CLA. Associations between SNPs and the VA and D9-desaturase traits were found on several chromosomes. A bioinformatics survey identified genes with an important role in pathways for milk fat and fatty acids metabolism within 1 Mb of SNP markers associated with fatty acids contents.
Bos taurus; genetic variability; milk fatty acids; pooled DNA
Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
Article (author)
File in questo prodotto:
File Dimensione Formato  
2014_Strillacci.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 778.89 kB
Formato Adobe PDF
778.89 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/238595
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 14
  • ???jsp.display-item.citation.isi??? 14
social impact