We have investigated the influence on adhesion and proliferation of NIH 3T3 fibroblasts of the surface morphology of cluster assembled carbon films deposited by Supersonic Cluster Beam Deposition. Nanostructured carbon films exhibit a multi-scale morphology, which resembles the surface structure of the extracellular matrix, and possess a high specific area, while being relatively smooth at all scales. Correlations between measured morphological parameters and adaptive cell response have been brought out. High specific area and smoothness appear to conceivably favour both the early attachment of plated cells and the long-term survival of adherent cells. Moreover, nano-structured carbon films affect the cells morphology as well as the extension and the number of the focal contacts.

Adhesion and proliferation of fibroblasts on cluster-assembled nanostructured carbon films : the role of surface morphology / C. Lenardi, C. Perego, V. Cassina, A. Podestà, A. D'Amico, D. Gualandris, S. Vinati, F. Fiorentini, G. Bongiorno, P.G.C. Piseri, V.F. Sacchi, P. Milani. - In: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY. - ISSN 1533-4880. - 6:12(2006), pp. 3718-3730.

Adhesion and proliferation of fibroblasts on cluster-assembled nanostructured carbon films : the role of surface morphology

C. Lenardi
Primo
;
C. Perego
Secondo
;
V. Cassina;A. Podestà;A. D'Amico;F. Fiorentini;P.G.C. Piseri;V.F. Sacchi
Penultimo
;
P. Milani
Ultimo
2006

Abstract

We have investigated the influence on adhesion and proliferation of NIH 3T3 fibroblasts of the surface morphology of cluster assembled carbon films deposited by Supersonic Cluster Beam Deposition. Nanostructured carbon films exhibit a multi-scale morphology, which resembles the surface structure of the extracellular matrix, and possess a high specific area, while being relatively smooth at all scales. Correlations between measured morphological parameters and adaptive cell response have been brought out. High specific area and smoothness appear to conceivably favour both the early attachment of plated cells and the long-term survival of adherent cells. Moreover, nano-structured carbon films affect the cells morphology as well as the extension and the number of the focal contacts.
Cell-substrate interactions ; Focal adhesion ; Nanostructured films ; Substrate topography ; Atomic force microscopy
Settore BIO/09 - Fisiologia
Settore FIS/01 - Fisica Sperimentale
Settore FIS/03 - Fisica della Materia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/23794
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 6
social impact