Uropathogenic Escherichia coli strain (UPEC) represents the most common cause of urinary tract infections that could affect both animals and humans. To fight this pathogen, enrofloxacin represent the election antibiotic, but there are considerable evidences that document how this microorganism is becoming more resistant to this antibiotic and it is developing multidrug resistance . Genomic characteristics of E. coli resistant to enrofloxacin have already been documented , however poor information have been provided about the molecular mechanisms of the resistant strains growing in presence of enrofloxacin. This work provides an interesting point of view about the mechanisms involved in E. coli isolates that developed resistance to enrofloxacin. E. coli UPEC isolates were obtained from urine of dogs with urinary infections, antibiotic resistance was induced with increasing concentrations of enrofloxacin up to 10 µg/ml. Obtained results showed the differential expression of respectively 19 (2D DIGE) and 56 (shotgun proteomics) proteins in E. coli resistant isolates against control group. To evaluate the functions and the pathways of the differentially expressed proteins data were analyzed through STRING and PANTHER. Most of the differentially expressed proteins were enzymes with catalytic activity. Analyzing the function of the differentially expressed proteins it was found in the resistant isolate a high overexpression of enzymes with reducing power and the overexpression of three different isoforms of a protein involved in the DNA protection. Since the enrofloxacin is an inhibitor of DNA gyrase the overexpression of DNA starvation/stationary phase protection protein Dsp could be very interesting to unravel the mechanisms of this bacteria to counteract the effects of enrofloxacin.
Mechanisms of enrofloxacin antibiotic resistance in zoonotic E. coli / C. Piras, A. Soggiu, V. Greco, L. Bonizzi, J. Nally, A. Urbani, P.A. Martino, M. Drigo, P. Roncada - In: 8th annual national conference : abstract volume / [a cura di] Italian Proteomics Association. - Padova : EdiSES, 2013. - ISBN 978-88-7959-775-3. - pp. 36-36 (( Intervento presentato al 8. convegno Annual national conference tenutosi a Padova nel 2013.
|Titolo:||Mechanisms of enrofloxacin antibiotic resistance in zoonotic E. coli|
|Parole Chiave:||Proteomics ; antibiotic resistance ; enrofloxacin ; Escherichia coli ; urine ; dogs|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Data di pubblicazione:||2013|
|Enti collegati al convegno:||ItPA|
Italian Proteomics Association
|Tipologia:||Book Part (author)|
|Appare nelle tipologie:||03 - Contributo in volume|