In male Wistar rats standardized from birth in LD 12:12 conditions (with light from 0700 to 1900 hr), samples of mucosa from the body of the stomach were isolated in vitro and mounted in a Ussing-type chamber. Spontaneous secretion of the hydrogen ion (H+)during 45 min of incubation was measured. Some electric parameters [transmucosal potential difference (PD) and electrical resistance (R)] were detected in the gastric mucosa both during H+ secretion and during inhibition of acid secretion by cimetidine. The variables studied were analyzed with the single cosinor method, and all revealed a circadian rhythm with acrophases during the dark span. The higher values of PD and R found during the dark hours are unrelated to acid secretion and may indicate a greater efficiency of ionic pumps and a limited passive permeability.
Circadian rhythms of acid secretion and electric parameters in rat gastric mucosa in vitro / U. Ventura, F. Carandente, E. Montini, C. Sforza, T. Ceriani, E. Iurlaro, R. Moggio - In: Advances in chronobiology : proceedings of the 17. international conference of the International society for chronobiology held in Little Rock, Arkansas, November 3-6, 1985. Part A / [a cura di] J.E. Pauly, L.E. Scheving. - New York : Liss, 1987. - ISBN 0845150774. - pp. 361-370 (( Intervento presentato al 17. convegno International Conference of the International Society for Chronobiology tenutosi a Little Rock, Arkansas nel 1985.
Circadian rhythms of acid secretion and electric parameters in rat gastric mucosa in vitro
F. CarandenteSecondo
;C. Sforza;
1987
Abstract
In male Wistar rats standardized from birth in LD 12:12 conditions (with light from 0700 to 1900 hr), samples of mucosa from the body of the stomach were isolated in vitro and mounted in a Ussing-type chamber. Spontaneous secretion of the hydrogen ion (H+)during 45 min of incubation was measured. Some electric parameters [transmucosal potential difference (PD) and electrical resistance (R)] were detected in the gastric mucosa both during H+ secretion and during inhibition of acid secretion by cimetidine. The variables studied were analyzed with the single cosinor method, and all revealed a circadian rhythm with acrophases during the dark span. The higher values of PD and R found during the dark hours are unrelated to acid secretion and may indicate a greater efficiency of ionic pumps and a limited passive permeability.
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