Silk fibroin scaffolds obtained via regeneration by Ajisawa's reagent (CaCl2/H2O/EtOH, mole ratio 1:8:2) and freeze-drying followed by steam sterilization resulted unstable when incubated in physiological medium over 1-month period since the oxygen diffusivity and mechanical resistance to compression significantly decreased. Blending of regenerated silk fibroin with PEG 600 lead to a novel scaffold with an improved water stability. Multi-parameter characterization has revealed that scaffolds made by composite material were stable in physiological media over at least 1month of incubation. Interestingly, the presence of PEG has a notable impact on wettability and protein adsorption. This increase in hydrophilicity could be beneficial for initial cell adhesion and survival as well as for the adsorptive loading of growth factors.

Enhanced hydration stability of Bombyx mori silk fibroin/PEG 600 composite scaffolds for tissue engineering / F. Selmin, C.G.M. Gennari, P. Minghetti, L.A. Marotta, B. Viviani, P. Vagdama, L. Montanari, F. Cilurzo. - In: POLYMERS FOR ADVANCED TECHNOLOGIES. - ISSN 1042-7147. - 25:5(2014), pp. 532-538.

Enhanced hydration stability of Bombyx mori silk fibroin/PEG 600 composite scaffolds for tissue engineering

F. Selmin
Primo
;
C.G.M. Gennari
Secondo
;
P. Minghetti;L.A. Marotta;B. Viviani;L. Montanari
Penultimo
;
F. Cilurzo
2014

Abstract

Silk fibroin scaffolds obtained via regeneration by Ajisawa's reagent (CaCl2/H2O/EtOH, mole ratio 1:8:2) and freeze-drying followed by steam sterilization resulted unstable when incubated in physiological medium over 1-month period since the oxygen diffusivity and mechanical resistance to compression significantly decreased. Blending of regenerated silk fibroin with PEG 600 lead to a novel scaffold with an improved water stability. Multi-parameter characterization has revealed that scaffolds made by composite material were stable in physiological media over at least 1month of incubation. Interestingly, the presence of PEG has a notable impact on wettability and protein adsorption. This increase in hydrophilicity could be beneficial for initial cell adhesion and survival as well as for the adsorptive loading of growth factors.
Composite materials; Mechanical properties; Scaffolds; Silk fibroin
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/235703
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