The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL-1 human chorionic gonadotrophin (hCG), 1?gmL-1 progesterone (P4) and 1?gmL-1 oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (PP<0.001). The stage of the oestrous cycle did not influence maturation rates.
|Titolo:||Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems|
LUVONI, GAIA CECILIA (Penultimo)
|Parole Chiave:||Dog; hormones; in vitro maturation|
|Settore Scientifico Disciplinare:||Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||10.1071/RD14022|
|Appare nelle tipologie:||01 - Articolo su periodico|