The influence of long-term copper contamination on the diversity of bacterial communities was investigated in an ex-vineyard soil. Two sites of the same area but exhibiting different 3-fold exchangeable copper (Ex-Cu) concentrations were analysed. Culturable bacterial community structure was assessed using a variety of approaches: determination of culturable bacteria number, analyses of 132 isolates, and denaturing gradient gel electrophoresis (DGGE) patterns of bacterial biomass grown on agar plates and of soil DNA. There was no significant difference in the number of total heterotrophs at the two sites, whereas the percentage of fast-growing bacteria growing in 1 day, was lower at the site with the higher Ex-Cu content. A high percentage of Cu-tolerant bacteria was found in both sites (63-70%) and it was relatively independent of the Cu content. Shifts in species composition of the culturable bacterial community were detected by analysing isolates from the two soils, Gram-positive bacteria prevailed in the less-polluted soil while Gram-negative bacteria in the more-polluted soil. Each sample site had a community with a different metal resistance pattern. Our study seems to indicate that in this soil ecosystem, copper influenced the culturable bacterial communities, affecting the structural diversity and altering some of the metal resistance of the microorganisms. The Sorensen similarity index calculated on DGGE profiles of 16S rDNA of total and culturable bacterial communities indicated a different species composition at the two sites, although both sites had the same biodiversity degree and different dominance.

Assessment of bacterial community structure in a long-term copper-polluted ex vineyard soil / M.E. Dell'Amico, M. Mazzocchi, L. Cavalca, L. Allievi, V. Andreoni. - In: MICROBIOLOGICAL RESEARCH. - ISSN 0944-5013. - 163:6(2008), pp. 671-683.

Assessment of bacterial community structure in a long-term copper-polluted ex vineyard soil

M.E. Dell'Amico
Primo
;
M. Mazzocchi
Secondo
;
L. Cavalca;L. Allievi
Penultimo
;
V. Andreoni
Ultimo
2008

Abstract

The influence of long-term copper contamination on the diversity of bacterial communities was investigated in an ex-vineyard soil. Two sites of the same area but exhibiting different 3-fold exchangeable copper (Ex-Cu) concentrations were analysed. Culturable bacterial community structure was assessed using a variety of approaches: determination of culturable bacteria number, analyses of 132 isolates, and denaturing gradient gel electrophoresis (DGGE) patterns of bacterial biomass grown on agar plates and of soil DNA. There was no significant difference in the number of total heterotrophs at the two sites, whereas the percentage of fast-growing bacteria growing in 1 day, was lower at the site with the higher Ex-Cu content. A high percentage of Cu-tolerant bacteria was found in both sites (63-70%) and it was relatively independent of the Cu content. Shifts in species composition of the culturable bacterial community were detected by analysing isolates from the two soils, Gram-positive bacteria prevailed in the less-polluted soil while Gram-negative bacteria in the more-polluted soil. Each sample site had a community with a different metal resistance pattern. Our study seems to indicate that in this soil ecosystem, copper influenced the culturable bacterial communities, affecting the structural diversity and altering some of the metal resistance of the microorganisms. The Sorensen similarity index calculated on DGGE profiles of 16S rDNA of total and culturable bacterial communities indicated a different species composition at the two sites, although both sites had the same biodiversity degree and different dominance.
No
English
Soil bacteria; Long-term copper pollution; Cu-resistant isolates
Settore AGR/16 - Microbiologia Agraria
Articolo
Esperti anonimi
Ricerca applicata
Pubblicazione scientifica
2008
Elsevier
163
6
671
683
13
Pubblicato
Periodico con rilevanza internazionale
Aderisco
info:eu-repo/semantics/article
Assessment of bacterial community structure in a long-term copper-polluted ex vineyard soil / M.E. Dell'Amico, M. Mazzocchi, L. Cavalca, L. Allievi, V. Andreoni. - In: MICROBIOLOGICAL RESEARCH. - ISSN 0944-5013. - 163:6(2008), pp. 671-683.
open
Prodotti della ricerca::01 - Articolo su periodico
5
262
Article (author)
si
M.E. Dell'Amico, M. Mazzocchi, L. Cavalca, L. Allievi, V. Andreoni
File in questo prodotto:
File Dimensione Formato  
Assessment.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 441.31 kB
Formato Adobe PDF
441.31 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/23511
Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 86
  • ???jsp.display-item.citation.isi??? 74
social impact