Susceptibility of 96 Listeria monocytogenes human isolates collected in northern Italy between 2008 and 2010, to 15 antimicrobials, was investigated. Minimum inhibitory concentration (MIC) was evaluated by means of the standardized broth microdilution method, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) international guidelines. All L. monocytogenes human isolates were susceptible to penicillin G (MIC90≤0·06 μg/ml), meropenem (MIC90≤0·06 μg/ml), and erythromycin (MIC90 = 0·12 μg/ml). Susceptibility to the other tested antimicrobials could not be interpreted due to the lack of breakpoint values although two (2%) isolates were shown to have tetracycline MICs above EUCAST epidemiological cut-off values (ECOFF). Bactericidal activity for amoxicillin, gentamicin, and levofloxacin was generally observed at concentrations 2-4 times higher than MIC values. Though L. monocytogenes human strains, isolated in the north of Italy, appear to be susceptible to most antimicrobial agents used in human therapy, this study provides new data for epidemiological surveillance and clinical breakpoints definition.

Antimicrobial susceptibility of Listeria monocytogenes isolates from human cases in northern Italy, 2008-2010: MIC determination according to EUCAST broth microdilution method / M. Madeo, R. Musumeci, A.M.L. Careddu, E. Amato, M.M. Pontello, C.E. Cocuzza. - In: JOURNAL OF CHEMOTHERAPY. - ISSN 1120-009X. - 27:4(2015 Aug), pp. 201-206. [10.1179/1973947814Y.0000000187]

Antimicrobial susceptibility of Listeria monocytogenes isolates from human cases in northern Italy, 2008-2010: MIC determination according to EUCAST broth microdilution method

M. Madeo
;
A.M.L. Careddu;E. Amato;M.M. Pontello
Penultimo
;
2015

Abstract

Susceptibility of 96 Listeria monocytogenes human isolates collected in northern Italy between 2008 and 2010, to 15 antimicrobials, was investigated. Minimum inhibitory concentration (MIC) was evaluated by means of the standardized broth microdilution method, according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) international guidelines. All L. monocytogenes human isolates were susceptible to penicillin G (MIC90≤0·06 μg/ml), meropenem (MIC90≤0·06 μg/ml), and erythromycin (MIC90 = 0·12 μg/ml). Susceptibility to the other tested antimicrobials could not be interpreted due to the lack of breakpoint values although two (2%) isolates were shown to have tetracycline MICs above EUCAST epidemiological cut-off values (ECOFF). Bactericidal activity for amoxicillin, gentamicin, and levofloxacin was generally observed at concentrations 2-4 times higher than MIC values. Though L. monocytogenes human strains, isolated in the north of Italy, appear to be susceptible to most antimicrobial agents used in human therapy, this study provides new data for epidemiological surveillance and clinical breakpoints definition.
Listeria monocytogenes; Antimicrobials susceptibility; Minimum inhibitory concentration; Bactericidal activity; EUCAST guidelines
Settore MED/42 - Igiene Generale e Applicata
11-apr-2014
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/234925
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