Temporally and spatially defined changes in cellular calcium (Ca(2+)) concentration represent stimulus-specific signals and regulate a myriad of biological processes. The development of ratiometric Ca(2+) reporter proteins like Yellow Cameleons (YCs) has greatly advanced our ability to analyze Ca(2+) dynamics in vivo with unprecedented spatial and temporal resolution. In plants, the application of these Ca(2+) reporter proteins has been pioneered for the investigation of Ca(2+) dynamics in guard cells, and recently their use has been extended to other single-cell models like growing pollen tubes and root hairs. However, in plants, the use of YC reporter proteins has largely remained restricted to the investigation of cytoplasmic alterations of Ca(2+) concentrations. Here, we provide an introduction to current methods for imaging Ca(2+) dynamics with increasing sophistication.
Ca2+ imaging in plants using genetically encoded Yellow Cameleon Ca2+ indicators / S. Behera, M. Krebs, G. Loro, K. Schumacher, A. Costa, J. Kudla. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2013:8(2013 Aug), pp. 700-703.
|Titolo:||Ca2+ imaging in plants using genetically encoded Yellow Cameleon Ca2+ indicators|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||ago-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1101/pdb.top066183|
|Appare nelle tipologie:||01 - Articolo su periodico|