Temporally and spatially defined changes in cellular calcium (Ca(2+)) concentration represent stimulus-specific signals and regulate a myriad of biological processes. The development of ratiometric Ca(2+) reporter proteins like Yellow Cameleons (YCs) has greatly advanced our ability to analyze Ca(2+) dynamics in vivo with unprecedented spatial and temporal resolution. In plants, the application of these Ca(2+) reporter proteins has been pioneered for the investigation of Ca(2+) dynamics in guard cells, and recently their use has been extended to other single-cell models like growing pollen tubes and root hairs. However, in plants, the use of YC reporter proteins has largely remained restricted to the investigation of cytoplasmic alterations of Ca(2+) concentrations. Here, we provide an introduction to current methods for imaging Ca(2+) dynamics with increasing sophistication.

Ca2+ imaging in plants using genetically encoded Yellow Cameleon Ca2+ indicators / S. Behera, M. Krebs, G. Loro, K. Schumacher, A. Costa, J. Kudla. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2013:8(2013 Aug), pp. 700-703. [10.1101/pdb.top066183]

Ca2+ imaging in plants using genetically encoded Yellow Cameleon Ca2+ indicators

G. Loro;A. Costa;
2013

Abstract

Temporally and spatially defined changes in cellular calcium (Ca(2+)) concentration represent stimulus-specific signals and regulate a myriad of biological processes. The development of ratiometric Ca(2+) reporter proteins like Yellow Cameleons (YCs) has greatly advanced our ability to analyze Ca(2+) dynamics in vivo with unprecedented spatial and temporal resolution. In plants, the application of these Ca(2+) reporter proteins has been pioneered for the investigation of Ca(2+) dynamics in guard cells, and recently their use has been extended to other single-cell models like growing pollen tubes and root hairs. However, in plants, the use of YC reporter proteins has largely remained restricted to the investigation of cytoplasmic alterations of Ca(2+) concentrations. Here, we provide an introduction to current methods for imaging Ca(2+) dynamics with increasing sophistication.
Settore BIO/04 - Fisiologia Vegetale
ago-2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/233682
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