The organization of the actin cytoskeleton is essential for several cellular processes. Here we report the characterization of a Saccharomyces cerevisiae novel gene, SDA1, encoding a highly conserved protein, which is essential for cell viability and is localized in the nucleus. Depletion or inactivation of Sda1 cause cell cycle arrest in G 1 by blocking both budding and DNA replication, without loss of viability. Furthermore, sda1-1 temperature-sensitive mutant cells arrest at the non-permissive temperature mostly without detectable structures of polymerized actin, although a normal actin protein level is maintained, indicating that Sda1 is required for proper organization of the actin cytoskeleton. To our knowledge, this is the first mutation shown to cause such a phenotype. Recovery of Sda1 activity restores proper assembly of actin structures, as well as budding and DNA replication. Furthermore we show that direct actin perturbation, either in sda1-1 or in cdc28-13 cells released from G 1 block, prevents recovery of budding and DNA replication. We also show that the block in G 1 caused by loss of Sda1 function is independent of Swe1. Altogether our results suggest that disruption of F-actin structure can block cell cycle progression in G 1 and that Sda1 is involved in the control of the actin cytoskeleton.

The Saccharomyces cerevisiae SDA1 gene is required for actin cytoskeleton organization and cell cycle progression / G. Buscemi, F. Saracino, D. Masnada, M.L. Carbone. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 113 ( Pt 7):7(2000 Apr), pp. 1199-1211.

The Saccharomyces cerevisiae SDA1 gene is required for actin cytoskeleton organization and cell cycle progression

G. Buscemi
Primo
;
2000

Abstract

The organization of the actin cytoskeleton is essential for several cellular processes. Here we report the characterization of a Saccharomyces cerevisiae novel gene, SDA1, encoding a highly conserved protein, which is essential for cell viability and is localized in the nucleus. Depletion or inactivation of Sda1 cause cell cycle arrest in G 1 by blocking both budding and DNA replication, without loss of viability. Furthermore, sda1-1 temperature-sensitive mutant cells arrest at the non-permissive temperature mostly without detectable structures of polymerized actin, although a normal actin protein level is maintained, indicating that Sda1 is required for proper organization of the actin cytoskeleton. To our knowledge, this is the first mutation shown to cause such a phenotype. Recovery of Sda1 activity restores proper assembly of actin structures, as well as budding and DNA replication. Furthermore we show that direct actin perturbation, either in sda1-1 or in cdc28-13 cells released from G 1 block, prevents recovery of budding and DNA replication. We also show that the block in G 1 caused by loss of Sda1 function is independent of Swe1. Altogether our results suggest that disruption of F-actin structure can block cell cycle progression in G 1 and that Sda1 is involved in the control of the actin cytoskeleton.
No
English
Actin; Cell cycle; Latrunculin-A; Saccharomyces cerevisiae
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
apr-2000
113 ( Pt 7)
7
1199
1211
13
Pubblicato
Periodico con rilevanza internazionale
info:eu-repo/semantics/article
The Saccharomyces cerevisiae SDA1 gene is required for actin cytoskeleton organization and cell cycle progression / G. Buscemi, F. Saracino, D. Masnada, M.L. Carbone. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - 113 ( Pt 7):7(2000 Apr), pp. 1199-1211.
open
Prodotti della ricerca::01 - Articolo su periodico
4
262
Article (author)
Periodico con Impact Factor
G. Buscemi, F. Saracino, D. Masnada, M.L. Carbone
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/233677
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