Protein over-expression in bacteria is still the easiest, cheapest and therefore preferred way to obtain large amounts of proteins for industrial and laboratory scale preparations. Several studies emphasized the importance of understanding cellular and molecular mechanisms triggered by protein over-production in order to obtain higher yield and better quality of the recombinant product. Almost every step leading to a fully functional polypeptide has been investigated, from mRNA stability to the role of molecular chaperones, from aggregation to bottlenecks in the secretory pathway. In this context, we focused on the still poorly addressed relationship between protein production in the cytoplasm and the bacterial envelope, an active and reactive cell compartment that controls interactions with the environment and several major cellular processes. Results available to date show that the accumulation of foreign proteins in the cytoplasm induces changes in the membrane lipids and in the levels of mRNAs for some membrane proteins. However, a direct connection between membrane protein expression levels and soluble/aggregated protein accumulation in the cytoplasm has never been reported.
|Titolo:||Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm|
VILLA, RICCARDO (Primo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore BIO/19 - Microbiologia Generale
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||10.1186/1475-2859-8-32|
|Appare nelle tipologie:||01 - Articolo su periodico|