Here we report a method for analyzing mitochondrial Ca2+ dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial- targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca2+dynamics within the same cell.
Imaging of mitochondrial and nuclear Ca2+ dynamics in Arabidopsis roots / A. Costa, G. Loro. - In: COLD SPRING HARBOR PROTOCOLS. - ISSN 1559-6095. - 2013:8(2013 Aug), pp. 781-785. [10.1101/pdb.prot073049]
Imaging of mitochondrial and nuclear Ca2+ dynamics in Arabidopsis roots
A. Costa;
2013
Abstract
Here we report a method for analyzing mitochondrial Ca2+ dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial- targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca2+dynamics within the same cell.
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