Crucial traits contributing to pathogenicity of Pseudomonas aeruginosa are fine-tuned by a dynamic and intricate regulatory network [1]. The involvement of small RNAs (sRNAs) in this virulence regulatory network has been underestimated, being so far less than ten the sRNAs shown to be implicated. We have recently performed a comparative sRNA profiling via deep-sequencing of P. aeruginosa [2]. We now aim to unravel virulence-relevant sRNA-based regulatory systems throughout the panel of novel sRNAs that we have identified. In this study, we have collected evidences for the involvement of the conserved sRNA SPA0122 in the regulation of motility and pyocyanin production. In addition, we suggest that SPA0122 negatively regulates the expression of a key enzyme providing sugar precursors for the synthesis of exopolysaccarides, and a protein involved in antibiotic susceptibility. Finally, we have evidences that SPA0122 is responsive to infection-relevant host stimuli such as oxygen availability and shift from room to body temperature. In conclusion, our results indicate that SPA0122, once receiving inputs from host sensing/signaling pathways, plays a role as global co-mediator of complex virulence regulatory sub-networks. 1. Balasubramanian et al., (2013) Nucleic Acids Res, 41:1-20. 2. Ferrara et al., (2012) PLoS One, 7:e36553.
Involvement of the conserved small RNA SPA0122 in the regulation of virulence traits of Pseudomonas aeruginosa / S. Carloni, R. Fulco, G. Bertoni, S. Ferrara. ((Intervento presentato al 30. convegno Microbiology 2013, 30° Meeting of SIMGBM - Società Italiana di Microbiologia Generale e Biotecnologie Microbiche tenutosi a Ischia nel 2013.
Involvement of the conserved small RNA SPA0122 in the regulation of virulence traits of Pseudomonas aeruginosa
S. Carloni;G. Bertoni;S. Ferrara
2013
Abstract
Crucial traits contributing to pathogenicity of Pseudomonas aeruginosa are fine-tuned by a dynamic and intricate regulatory network [1]. The involvement of small RNAs (sRNAs) in this virulence regulatory network has been underestimated, being so far less than ten the sRNAs shown to be implicated. We have recently performed a comparative sRNA profiling via deep-sequencing of P. aeruginosa [2]. We now aim to unravel virulence-relevant sRNA-based regulatory systems throughout the panel of novel sRNAs that we have identified. In this study, we have collected evidences for the involvement of the conserved sRNA SPA0122 in the regulation of motility and pyocyanin production. In addition, we suggest that SPA0122 negatively regulates the expression of a key enzyme providing sugar precursors for the synthesis of exopolysaccarides, and a protein involved in antibiotic susceptibility. Finally, we have evidences that SPA0122 is responsive to infection-relevant host stimuli such as oxygen availability and shift from room to body temperature. In conclusion, our results indicate that SPA0122, once receiving inputs from host sensing/signaling pathways, plays a role as global co-mediator of complex virulence regulatory sub-networks. 1. Balasubramanian et al., (2013) Nucleic Acids Res, 41:1-20. 2. Ferrara et al., (2012) PLoS One, 7:e36553.Pubblicazioni consigliate
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