Specific and non-specific complexes of DNA and photolyase are visualised by atomic force microscopy. As a substrate for photolyase a 1150 bp DNA restriction fragment was UV-irradiated to produce damaged sites at random positions. Comparison with a 735 bp undamaged DNA fragment made it possible to separate populations of specific and non-specific photolyase complexes on the 1150 bp fragment, relieving the need for highly defined substrates. Thus it was possible to compare DNA bending for specific and non-specific interactions. Non-specific complexes show no significant bending but increased rigidity compared to naked DNA, whereas specific complexes show DNA bending of on average 36° and higher flexibility. A model obtained by docking shows that photolyase can accommodate a 36° bent DNA in the vicinity of the active site.
|Titolo:||DNA bending by photolyase in specific and non-specific complexes studied by atomic force microscopy|
|Settore Scientifico Disciplinare:||Settore FIS/03 - Fisica della Materia|
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore FIS/01 - Fisica Sperimentale
|Data di pubblicazione:||1999|
|Digital Object Identifier (DOI):||10.1093/nar/27.19.3875|
|Appare nelle tipologie:||01 - Articolo su periodico|