To identify molecular targets that interact with zosteric acid, a small-scale affinity purification of proteins from E. coli extracts on immobilized zosteric acid moieties was performed. According to the biological screening of the small library of molecules, p-aminocinnamic acid was selected for its covalent coupling to the affinity support. Functionalization of the solid support was successfully demonstrated by fluorescence analysis. The protein pull-down revealed that one protein bound to cinnamate coupled matrix. The identification of this protein is still in progress.
|Titolo:||Antibiofilm activity of selected natural compounds|
CATTO', CRISTINA (Primo)
|Data di pubblicazione:||gen-2014|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
Settore AGR/16 - Microbiologia Agraria
|Citazione:||Antibiofilm activity of selected natural compounds / C. Cattò. ((Intervento presentato al 9. convegno Workshop. Doctorate in Chemistry, Biochemistry and Ecology of Plant Protection Products and Xenobiotics tenutosi a Milano nel 2014.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|