Synaptosomal-associated protein of 25 kDa (SNAP-25) is a member of the Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) protein family, required for exocytosis of synaptic vesicles and regulation of diverse ion channels. Here, we show that acute reduction of SNAP-25 expression leads to an immature phenotype of dendritic spines that are, consistently, less functional. Conversely, over-expression of SNAP-25 results in an increase in the density of mature, Postsynaptic Density protein 95 (PSD-95)-positive spines. The regulation of spine morphogenesis by SNAP-25 depends on the protein's ability to bind both the plasma membrane and the adaptor protein p140Cap, a key protein regulating actin cytoskeleton and spine formation. We propose that SNAP-25 allows the organization of the molecular apparatus needed for spine formation by recruiting and stabilizing p140Cap.
|Titolo:||SNAP-25 regulates spine formation through postsynaptic binding to p140Cap|
|Parole Chiave:||gene expression regulation, developmental; actin cytoskeleton; adaptor proteins, vesicular transport; animals; dendritic spines; embryo, mammalian; hela cells; hippocampus; humans; intracellular signaling peptides and proteins; membrane proteins; primary cell culture; protein binding; protein stability; rats; signal transduction; synaptosomal-associated protein 25|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2013|
|Digital Object Identifier (DOI):||10.1038/ncomms3136|
|Appare nelle tipologie:||01 - Articolo su periodico|