Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E. coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presented DC-derived IL-15 contribute equally to optimal NK cell activation.
IL-15 cis presentation is required for optimal NK cell activation in lipopolysaccharide-mediated inflammatory conditions / I. Zanoni, R. Spreafico, C. Bodio, M. Di Gioia, C. Cigni, A. Broggi, T. Gorletta, M. Caccia, G. Chirico, L. Sironi, M. Collini, M.P. Colombo, N. Garbi, F. Granucci. - In: CELL REPORTS. - ISSN 2211-1247. - 4:6(2013 Sep 26), pp. 1235-1249.
|Titolo:||IL-15 cis presentation is required for optimal NK cell activation in lipopolysaccharide-mediated inflammatory conditions|
|Parole Chiave:||IL-15 ; dendritic cells ; nk cells ; innate immunity ; cytokines|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||26-set-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.celrep.2013.08.021|
|Appare nelle tipologie:||01 - Articolo su periodico|