Human adipose tissue – derived stem cells (hADSCs) are routinely isolated from the stromal vascular fraction of homogenized adipose tissue. In this study a mechanical, non-enzymatic method to obtain mesenchymal stem cells from the human subcutaneous adipose tissue, by use of the Lipogems® device has been set. This instrument allows three micro-fragmentation steps of the lipoaspirate adipose tissue, in order to increase the stem cells bioavailability, residents in the stromal vascular fraction of the tissue, diminishing oil and blood residues. The structural composition of the Lipogems® product is comparable to the original lipoaspirate adipose tissue, but differently, this mechanical procedure activates the expression of antigens such as β-tubulin III. The Lipogems® product could be processed to obtain hADSCs with different approaches, including the classical enzymatic treatment, a single centrifugation or a directly tissue culture, although the yield is different and it is associated to the used method. Freshly isolated they display surface markers that differ from those of cultured hADSCs. The hADSCs derived from Lipogems® product have been systematically characterized for growth features, phenotype and multipotent differentiation potential. They fulfill the definition of mesenchymal stem cells provided by the International Society of Cell Theraphy (ISCT). Notably, in basal growth condition Lipogems®- derived stem cells express at high percentages typical neural markers, such as GFAP, O4 and β-tubulin III. The EPO Receptor (EPOR) is express when these cells grown in SCM medium. All these findings provide evidence that micro-fragmentation of lipoaspirate adipose tissue change some molecular features in the tissue resident cells so that hADSCs obtained in this condition become more plastic and adaptive to the extracellular signals. The immunomodulatory properties of Lipogems®- derived stem cells have been evaluated by means of anti CD3 assay of co-culture with CD3+ T lymphocytes. They show a strongly suppressive effect on the T cells proliferation, maintaining a consistent T regulatory population.

MESENCHYMAL STEM CELLS FROM HUMAN ADIPOSE TISSUE:CHARACTERIZATION AND IMMUNOMODULATORY PROPERTIES OF TISSUE AND CELLULAR PRODUCT / F. Messaggio ; tutor: A. Gorio ; coordinatore: A. Gorio. - : . Università degli Studi di Milano, 2014 Jan 30. ((26. ciclo, Anno Accademico 2013. [10.13130/messaggio-fanuel_phd2014-01-30].

MESENCHYMAL STEM CELLS FROM HUMAN ADIPOSE TISSUE:CHARACTERIZATION AND IMMUNOMODULATORY PROPERTIES OF TISSUE AND CELLULAR PRODUCT

F. Messaggio
2014-01-30

Abstract

Human adipose tissue – derived stem cells (hADSCs) are routinely isolated from the stromal vascular fraction of homogenized adipose tissue. In this study a mechanical, non-enzymatic method to obtain mesenchymal stem cells from the human subcutaneous adipose tissue, by use of the Lipogems® device has been set. This instrument allows three micro-fragmentation steps of the lipoaspirate adipose tissue, in order to increase the stem cells bioavailability, residents in the stromal vascular fraction of the tissue, diminishing oil and blood residues. The structural composition of the Lipogems® product is comparable to the original lipoaspirate adipose tissue, but differently, this mechanical procedure activates the expression of antigens such as β-tubulin III. The Lipogems® product could be processed to obtain hADSCs with different approaches, including the classical enzymatic treatment, a single centrifugation or a directly tissue culture, although the yield is different and it is associated to the used method. Freshly isolated they display surface markers that differ from those of cultured hADSCs. The hADSCs derived from Lipogems® product have been systematically characterized for growth features, phenotype and multipotent differentiation potential. They fulfill the definition of mesenchymal stem cells provided by the International Society of Cell Theraphy (ISCT). Notably, in basal growth condition Lipogems®- derived stem cells express at high percentages typical neural markers, such as GFAP, O4 and β-tubulin III. The EPO Receptor (EPOR) is express when these cells grown in SCM medium. All these findings provide evidence that micro-fragmentation of lipoaspirate adipose tissue change some molecular features in the tissue resident cells so that hADSCs obtained in this condition become more plastic and adaptive to the extracellular signals. The immunomodulatory properties of Lipogems®- derived stem cells have been evaluated by means of anti CD3 assay of co-culture with CD3+ T lymphocytes. They show a strongly suppressive effect on the T cells proliferation, maintaining a consistent T regulatory population.
GORIO, ALFREDO
GORIO, ALFREDO
Settore BIO/14 - Farmacologia
MESENCHYMAL STEM CELLS FROM HUMAN ADIPOSE TISSUE:CHARACTERIZATION AND IMMUNOMODULATORY PROPERTIES OF TISSUE AND CELLULAR PRODUCT / F. Messaggio ; tutor: A. Gorio ; coordinatore: A. Gorio. - : . Università degli Studi di Milano, 2014 Jan 30. ((26. ciclo, Anno Accademico 2013. [10.13130/messaggio-fanuel_phd2014-01-30].
Doctoral Thesis
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/230544
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