Costruction of a molecular switchboard by assembling ribonucleopeptide biosensors

Herein, we report the construction of a molecular switchboard based on the assembly of ATP-binding ribonucleopeptide (RNP) biosensors on DNA origami. ATP-binding fluorescent biosensors based on RNP havebeen developed from RNP receptors obtained by in vitro selection. In order to locate RNP biosensors on DNA origami surface, a tag-specific sequence was introduced at the 5'- end of RNA subunit and a covalent bond was introduced between the 3'-end of recognition RNA module and the peptide reporter module. ATP sensors were selectively attached trough hybridization on 2D origami structures and detected by AFM.