We present here a new methodology for quantitative mapping of light elements in cells, based on combination of compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Microscopy and Scanning Transmission X-ray Microscopy (STXM). Since XRFM of light elements (carbon, nitrogen, oxygen, sodium and magnesium), are strongly influenced by self-absorption, we developed an algorithm to correct for this effect, using the morphological and structural information provided by AFM and STXM. Finally, the corrected distributions have been obtained, thus allowing quantitative mapping.
|Titolo:||X-ray fluorescence microscopy of light elements in cells: self-absorption correction by integration of compositional and morphological measurements|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||2013|
|Digital Object Identifier (DOI):||10.1088/1742-6596/463/1/012022|
|Appare nelle tipologie:||01 - Articolo su periodico|