A large complex of proteins, called CENPs, are associated with centromeric DNA. Some of them exhibit a cell cycle-related expression (e.g., CENP-E and -F) and are required for the transition from interphase to mitosis, whereas constitutive proteins (e.g., CENP-A, -B, -C, -G, and -H) reside permanently at the centromere and are essential for the correct kinetochore assembly. Poly(ADP-ribose) polymerase-1 (PARP-1), which plays an active role in many basic processes, was described as a possible regulator of CENPs. By multicolor immunofluorescence we therefore analyzed the distribution of PARP-1 and its interaction with CENP-B, -E, and -F during mitosis and apoptosis.
|Titolo:||Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis|
PERDONI, FEDERICA (Primo)
|Parole Chiave:||Apoptosis; CENPs; Fluorescence microscopy; Immunocytochemistry; Mitosis; PARP-1|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||ago-2009|
|Digital Object Identifier (DOI):||10.1111/j.1749-6632.2009.04729.x|
|Appare nelle tipologie:||01 - Articolo su periodico|