Background: Many biomaterials are proposed for sinus floor lifting and grafting in the posterior maxilla for insufficient bone volume (BV). The aim of this study was to compare the use of magnesium-enriched hydroxyap-atite (mHA) versus autogenous bone graft (AB) for maxillary sinus lift procedures by histomorphometric and in vivo gene expression profiling. Methods: Fifteen patients requiring bilateral maxillary sinus augmentation received autologous bone particles (group A) and mHA (group B) (split-mouth design). Five months later, implants were placed, and biopsies were obtained. Bone specimens were analyzed by histomorphometry, BV and vital bone (VB) percentages were calculated, and ex vivo osteoblast expansion followed by highly sensitive osteoblast specific gene expression profiling for cbfal, osteocalcin, osteopontin, collagen type I, receptor activator of nuclear factor-kappa B ligand (RANKL), and osteo-protegerin (OPG) by quantitative real-time reverse transcription-polymer-ase chain reaction (RT-PCR) were performed. Comparisons were made using the Student t test. Results: After healing with no complications, B V was comparable in the two groups (80.79% ± 14.27% for autologous versus 76,72% ±11,47% for mHA; P= not statistically significant), but VB was lower in the mHA group (29.65% + 9.81% versus 78.40% ± 16.72%; P<0.05). Real-time RT-PCR analyses showed significantly higher expression of the osteoblast differentiation factor Cbfal and the matrix formation marker osteocalcin in the mHA group compared to the AB group, whereas type I collagen was comparable, and osteopontin was decreased. Attesting to a lower osteoclasto-genic potential, the RANKL/OPQ ratio was diminished. Conclusions: Autogenous bone samples provided higher vital over comparable total bone levels than mHA-grafted sites. Osteoblast gene expression profiles from mHA grafts revealed higher expression of certain specific markers of osteoblast differentiation and bone formation, associated with a lower osteoclastogenic potential.
|Titolo:||Magnesium-enriched hydroxyapatite versus autologous bone in maxillary sinus grafting : combining histomorphometry with osteoblast gene expression profiles ex vivo|
|Parole Chiave:||Bone Regeneration ; Bone Substitutes ; Bone Transplantation ; Collagen Type I ; Core Binding Factor Alpha 1 Subunit ; Durapatite ; Female ; Gene Expression Profiling ; Humans ; Magnesium ; Male ; Maxillary Sinus ; Middle Aged ; Oral Surgical Procedures, Preprosthetic ; Osteoblasts ; Osteocalcin ; Osteopontin ; Osteoprotegerin ; RANK Ligand ; Reverse Transcriptase Polymerase Chain Reaction|
|Settore Scientifico Disciplinare:||Settore MED/33 - Malattie Apparato Locomotore|
|Data di pubblicazione:||apr-2009|
|Digital Object Identifier (DOI):||10.1902/jop.2009.080466|
|Appare nelle tipologie:||01 - Articolo su periodico|