Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.
Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis / L. Polonelli, G. Morace. - In: JOURNAL OF CLINICAL MICROBIOLOGY. - ISSN 0095-1137. - 21:1(1985 Jan), pp. 138-139.
Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis
G. MoraceUltimo
1985
Abstract
Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.File | Dimensione | Formato | |
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