A crystal structure is reported for the C168S mutant of a typical 2-Cys peroxiredoxin III (Prx III) from bovine mitochondria at a resolution of 3.3 Å. Prx III is present as a two-ring catenane comprising two interlocking dodecameric toroids that are assembled from basic dimeric units. Each ring has an external diameter of 150 Å and encompasses a central cavity that is 70 Å in width. The concatenated dodecamers are inclined at an angle of 55°, which provides a large contact surface between the rings. Dimer-dimer contacts involved in toroid formation are hydrophobic in nature, whereas the 12 areas of contact between interlocked rings arise from polar interactions. These two major modes of subunit interaction provide important insights into possible mechanisms of catenane formation.

Bovine mitochondrial peroxiredoxin III forms a two-ring catenane / Z. Cao, A.W. Roszak, L.J. Gourlay, J.G. Lindsay, N.W. Isaacs. - In: STRUCTURE. - ISSN 0969-2126. - 13:11(2005), pp. 1661-1664.

Bovine mitochondrial peroxiredoxin III forms a two-ring catenane

L.J. Gourlay;
2005

Abstract

A crystal structure is reported for the C168S mutant of a typical 2-Cys peroxiredoxin III (Prx III) from bovine mitochondria at a resolution of 3.3 Å. Prx III is present as a two-ring catenane comprising two interlocking dodecameric toroids that are assembled from basic dimeric units. Each ring has an external diameter of 150 Å and encompasses a central cavity that is 70 Å in width. The concatenated dodecamers are inclined at an angle of 55°, which provides a large contact surface between the rings. Dimer-dimer contacts involved in toroid formation are hydrophobic in nature, whereas the 12 areas of contact between interlocked rings arise from polar interactions. These two major modes of subunit interaction provide important insights into possible mechanisms of catenane formation.
Animals ; Catenanes ; Cattle ; Crystallography, X-Ray ; Cysteine ; Dimerization ; Mitochondrial Proteins ; Mutation ; Peroxiredoxins ; Protein Structure, Quaternary ; Protein Structure, Tertiary
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/227881
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