Expression plasmids for use in Candida glabrata

We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata. The set of promoters includes three constitutive promoters (EGD2pr, HHT2pr, PDC1pr), two macrophage/phagocytosis-induced promoters (ACO2pr, LYS21pr), and one nutritionally regulated promoter (MET3pr). Each promoter was cloned into two plasmid backbones which differ in their selectable marker - URA3, or the dominant-selectable NAT1 gene, which confers resistance to the drug nourseothricin (Nat). Expression from the twelve resulting plasmids was assessed using GFP as a reporter and flow cytometry or quantitative reverse-transcription PCR (RTqPCR) to assess expression levels. Together this set of plasmids expands the toolkit of expression vectors available for use in C. glabrata.