The effect of in vitro cadmium exposure on catfish natural killer (NK) cells was investigated. Purification of NK cells by nylon-wool columns and density gradient centrifugation indicated that they were a population of nylon-wool adherent cells. Subsequently NK cells were separated only by density gradient centrifugation, where these cells band at the 43/45·5% Percoll interface. In some cytotoxicity assays, catfish NK cells were exposed to 5, 10, 30 and 50 μM cadmium (CdCl2); 10 μl of Cd solutions were added to NK cells simultaneously with the target cells. In vitro, 5 μM Cd inhibited the ability of catfish NK cells to kill the human erythroleukemic cell line K-562. In the presence of 10 μM Cd, the antibody-independent cytotoxic activity was totally suppressed.
|Titolo:||Effect of in vitro cadmium exposure on natural killer (NK) cells of catfish, Ictalurus melas|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||1996|
|Digital Object Identifier (DOI):||10.1006/fsim.1996.0017|
|Appare nelle tipologie:||01 - Articolo su periodico|