We evaluated the sensitivity of a DNA amplification test for the detection of Mycobacterium avium in blood samples using different blood components and different DNA extraction methods. M. avium-inoculated blood samples were processed to obtain separate blood components: peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNCs), and whole-blood sodium dodecyl sulfate (SDS)-lysate pellets. The sensitivity for the detection of the lowest mycobacterial load (1 CFU/ml) was significantly greater (P < 0.01) with DNA extracted from SDS-lysate pellets than with DNA extracted from PBMCs or PMNCs. Subsequently, DNA extraction methods based on guanidine NaOH, and proteinase were compared. The sensitivity of the guanidine-based method was significantly greater (P < 0.01) than those of the others.
|Titolo:||PCR-hybridization assay for Mycobacterium avium complex: optimization of detection in peripheral blood from humans|
|Parole Chiave:||human-immunodeficiency-virus; polymerase chain-reaction; clinical specimens; identification; tuberculosis; bacteremia; infection; disease; AIDS; diagnosis|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
|Data di pubblicazione:||apr-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1128/JCM.39.4.1638-1643.2001|
|Appare nelle tipologie:||01 - Articolo su periodico|