Nanostructured cubic zirconia is a strategic material for biomedical applications since it combines superior structural and optical properties with a nanoscale morphology able to control cell adhesion and proliferation. We produced nanostructured cubic zirconia thin films at room temperature by supersonic cluster beam deposition of nanoparticles produced in the gas phase. Precise control of film roughness at the nanoscale is obtained by operating in a ballistic deposition regime. This allows one to study the influence of nanoroughness on cell adhesion, while keeping the surface chemistry constant. We evaluated cell adhesion on nanostructured zirconia with an osteoblast-like cell line using confocal laser scanning microscopy for detailed morphological and cytoskeleton studies. We demonstrated that the organization of cytoskeleton and focal adhesion formation can be controlled by varying the evolution of surface nanoroughness.

Bottom-up engineering of the surface roughness of nanostructured cubic zirconia to control cell adhesion / M. Ferri, M. Tamplenizza, F. Borghi, G. Divitini, C. Ducati, C. Lenardi, C. Piazzoni, M. Merlini, A. Podestà, P. Milani. - In: NANOTECHNOLOGY. - ISSN 0957-4484. - 23:47(2012 Nov 30), pp. 475101.1-475101.10.

Bottom-up engineering of the surface roughness of nanostructured cubic zirconia to control cell adhesion

F. Borghi;C. Lenardi;C. Piazzoni;M. Merlini;A. Podestà;P. Milani
2012-11-30

Abstract

Nanostructured cubic zirconia is a strategic material for biomedical applications since it combines superior structural and optical properties with a nanoscale morphology able to control cell adhesion and proliferation. We produced nanostructured cubic zirconia thin films at room temperature by supersonic cluster beam deposition of nanoparticles produced in the gas phase. Precise control of film roughness at the nanoscale is obtained by operating in a ballistic deposition regime. This allows one to study the influence of nanoroughness on cell adhesion, while keeping the surface chemistry constant. We evaluated cell adhesion on nanostructured zirconia with an osteoblast-like cell line using confocal laser scanning microscopy for detailed morphological and cytoskeleton studies. We demonstrated that the organization of cytoskeleton and focal adhesion formation can be controlled by varying the evolution of surface nanoroughness.
cluster beam deposition; stabilized zirconia; ceramics; technology; oxide
Settore FIS/03 - Fisica della Materia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/226347
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