PURPOSE: Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials. METHODS: S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine. RESULTS: N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aeruginosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy. CONCLUSIONS: The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.
Activity of N-acetyl-L-cysteine against biofilm of Staphylococcus aureus and Pseudomonas aeruginosa on orthopedic prosthetic materials / L. Drago, E. De Vecchi, R. Mattina, C. Romanò. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 36:1(2013 Jan), pp. 39-46.
|Titolo:||Activity of N-acetyl-L-cysteine against biofilm of Staphylococcus aureus and Pseudomonas aeruginosa on orthopedic prosthetic materials|
DRAGO, LORENZO (Primo)
MATTINA, ROBERTO (Penultimo)
|Parole Chiave:||Bacterial biofilm; N-acetyl-L-cysteine; Prosthetic joint infections; Pseudomonas aeruginosa; Staphylococcus aureus|
|Settore Scientifico Disciplinare:||Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||gen-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.5301/ijao.5000135|
|Appare nelle tipologie:||01 - Articolo su periodico|