Apple proliferation (AP), caused by ‘Candidatus Phytoplasma mali’, is one of the most important phytoplasma diseases in Europe. So far, due to the absence of AP resistant varieties, the management of AP mainly consists in insecticide treatment against the insect vectors and in the eradication of diseased plants. These treatments have a strong economic and environmental effect, representing a risk for both operators and final customers. One of the most innovative solutions to develop sustainable approaches is the use of endophytes as inducers of the natural plant defense responses. In this work, we describe and characterized the endophytic bacterial community associated with healthy and AP phytoplasma-infected apple roots. Endophytic bacterial diversity associated with these plants was analyzed by cultivation-dependent and –independent methods. Sequence analysis of 16S rRNA gene libraries showed the presence of Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chlamydiae, and Firmicutes. The endophytic bacteria isolated on culture media were characterized for five beneficial traits related to mineral nutrition (phosphate solubilization, siderophores, nitrogen fixation), development (indolacetic acid synthesis), stress relief (catalase activity), disease control (siderophores). On the basis of this data some putative biocontrol strains (e.g. Burkholderia sp. and Pantoea sp.) were transformed for the production of fluorescent proteins in order to study the colonization pattern and their cycle inside apple plants. Pantoea sp.: pRL67511prfp successfully colonized the interior part of the plant and survived at least on month inside the tissues. Future studies will be carried out to investigate the ability of this endophyte to control apple proliferation.

Characterization of beneficial endophytic bacteria isolated from healthy and 'Candidatus Phytoplasma mali' infected plants / D. Bulgari, P. Casati, F. Quaglino, P.A. Bianco. ((Intervento presentato al 19. convegno Convegno Nazionale SIPAV tenutosi a Padova nel 2013.

Characterization of beneficial endophytic bacteria isolated from healthy and 'Candidatus Phytoplasma mali' infected plants

D. Bulgari;P. Casati;F. Quaglino
Penultimo
;
P.A. Bianco
2013-09

Abstract

Apple proliferation (AP), caused by ‘Candidatus Phytoplasma mali’, is one of the most important phytoplasma diseases in Europe. So far, due to the absence of AP resistant varieties, the management of AP mainly consists in insecticide treatment against the insect vectors and in the eradication of diseased plants. These treatments have a strong economic and environmental effect, representing a risk for both operators and final customers. One of the most innovative solutions to develop sustainable approaches is the use of endophytes as inducers of the natural plant defense responses. In this work, we describe and characterized the endophytic bacterial community associated with healthy and AP phytoplasma-infected apple roots. Endophytic bacterial diversity associated with these plants was analyzed by cultivation-dependent and –independent methods. Sequence analysis of 16S rRNA gene libraries showed the presence of Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chlamydiae, and Firmicutes. The endophytic bacteria isolated on culture media were characterized for five beneficial traits related to mineral nutrition (phosphate solubilization, siderophores, nitrogen fixation), development (indolacetic acid synthesis), stress relief (catalase activity), disease control (siderophores). On the basis of this data some putative biocontrol strains (e.g. Burkholderia sp. and Pantoea sp.) were transformed for the production of fluorescent proteins in order to study the colonization pattern and their cycle inside apple plants. Pantoea sp.: pRL67511prfp successfully colonized the interior part of the plant and survived at least on month inside the tissues. Future studies will be carried out to investigate the ability of this endophyte to control apple proliferation.
Settore AGR/12 - Patologia Vegetale
Characterization of beneficial endophytic bacteria isolated from healthy and 'Candidatus Phytoplasma mali' infected plants / D. Bulgari, P. Casati, F. Quaglino, P.A. Bianco. ((Intervento presentato al 19. convegno Convegno Nazionale SIPAV tenutosi a Padova nel 2013.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/226004
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