Rare bleeding disorders (RBDs) are a heterogeneous group of diseases with varying bleeding tendency, only partially explained by their laboratory phenotype. We analysed the separate groups of RBD abnormalities, and we investigated retrospectively whether the novel haemostasis assay (NHA) was able to differentiate between bleeding tendency. We have performed simultaneous thrombin generation (TG) and plasmin generation (PG) measurements in 41 patients affected with deficiencies in prothrombin, factor (F) V, FVII, FX, FXIII and fibrinogen. Parameters of the NHA were classified based on (major or minor) bleeding tendency. Patients with deficiencies in coagulation propagation (FII, FV and FX) and major type of bleedings had diminished TG (expressed as AUC) below 20% of control. FVII deficient patients only had prolonged thrombin lag-time ratio of 1.6 ± 0.2 (P < 0.05) and normal AUC (92-125%). Afibrinogenemic patients demonstrated PG of 2-29% of normal and appeared to correlate with the type of mutation. Thrombin peak-height (57 ± 16%) was reduced (not significant) in these patients and AUC was comparable to the reference (102 ± 27%). FXIII-deficient plasmas resulted in a reduced thrombin peak-height of 59 ± 13% (P < 0.05) and normal AUC (90 ± 14%). Thrombin peak-height (P < 0.01) and plasmin potential (P < 0.05) were lower in the major bleeders compared with the minor bleeders. These results provided distinct TG and PG curves for each individual abnormality and differentiation of bleeding tendency was observed for thrombin and PG parameters. Prospective studies are warranted to confirm these retrospective results.
Retrospective evaluation of bleeding tendency and simultaneous thrombin and plasmin generation in patients with rare bleeding disorders / M. Van Geffen, M. Menegatti, A. Loof, P. Lap, M. Karimi, B.A. Laros-van Gorkom, P. Brons, W.L. Van Heerde. - In: HAEMOPHILIA. - ISSN 1351-8216. - 18:4(2012 Jul), pp. 630-638.
|Titolo:||Retrospective evaluation of bleeding tendency and simultaneous thrombin and plasmin generation in patients with rare bleeding disorders|
MENEGATTI, MARZIA (Secondo)
|Parole Chiave:||Adolescent ; adult ; aged ; blood coagulation disorders ; cohort studies ; female ; fibrinolysin ; hemorrhage ; humans ; male ; middle aged ; predictive value of tests ; retrospective studies ; thrombin ; young adult|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||lug-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1365-2516.2012.02759.x|
|Appare nelle tipologie:||01 - Articolo su periodico|