We evaluated the sensitivity and specificity of a nested polymerase chain reaction (PCR) to the Mycobacterium tuberculosis IS6110 sequence on formalin-fixed paraffin-embedded tissue samples from patients with tubercular and other granulomatous lesions. Five groups of patients and samples were studied: (1) 28 samples from HIV-positive patients with tuberculosis, (2) 8 samples from HIV-negative patients with histologically suspected tuberculosis (confirmed by culture in 5 cases), (3) lymph nodes from 5 HIV-positive patients with Mycobacterium avium-intracellulare infection, (4) lymph nodes from 30 patients with sarcoidosis, and (5) specimens from 17 patients with other granulomatous diseases. The DNA was extracted from sections with a total thickness of 60 microm, and PCR amplified an internal fragment of 123 base pairs. All of the cases with M. tuberculosis infection were PCR-positive, although this sensitivity was partially related to the initial concentration of the DNA used for amplification. Two of the group 4 samples also were repeatedly positive, thus reducing the specificity of the method. All of the cases with granulomatous diseases other than sarcoidosis were negative. We propose a simplified and highly sensitive nested PCR for the diagnosis of M. tuberculosis infection on archived material in HIV-positive and HIV-negative patients.
Nested polymerase chain reaction for mycobacterium tuberculosis IS6110 sequence on formalin-fixed paraffin-embedded tissues with granulomatous diseases for rapid diagnosis of tuberculosis / L. Vago, M. Barberis, A. Gori, P. Scarpellini, E. Sala, M. Nebuloni, S. Bonetto, M. Cannone, G. Marchetti, F. Franzetti, G. Costanzi. - In: AMERICAN JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0002-9173. - 109:4(1998 Apr), pp. 411-415.
|Titolo:||Nested polymerase chain reaction for mycobacterium tuberculosis IS6110 sequence on formalin-fixed paraffin-embedded tissues with granulomatous diseases for rapid diagnosis of tuberculosis|
VAGO, GIANLUCA GAETANO (Corresponding)
COSTANZI, GIULIO (Ultimo)
|Parole Chiave:||tuberculosis; polymerase chain reaction [PCR]; HIV; AIDS; sarcoidosis|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
Settore MED/08 - Anatomia Patologica
|Data di pubblicazione:||apr-1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/ajcp/109.4.411|
|Appare nelle tipologie:||01 - Articolo su periodico|