Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.

The genome sequence of the colonial chordate, Botryllus schlosseri / A. Voskoboynik, N.F. Neff, S. Debashis, A.M. Newman, D. Pushkarev, W. Koh, B. Passarelli, H.C. Fan, G.L. Mantalas, K.J. Palmeri, K.J. Ishizuka, C. Gissi, F. Griggio, R. Ben-Shlomo, D.M. Corey, L. Penland, R.A. White, I.L. Weissman, S.R. Quake. - In: ELIFE. - ISSN 2050-084X. - 2:2(2013 Jul), pp. e00569.1-e00569.24.

The genome sequence of the colonial chordate, Botryllus schlosseri

C. Gissi;F. Griggio;
2013

Abstract

Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration.
Botryllus schlosseri; Other; genome; hematopoiesis; stem cell; tunicates; vertebrate evolution
Settore BIO/11 - Biologia Molecolare
lug-2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/223879
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