Physoplexis comosa and Primula glaucescens, endemic to calcareous grasslands of the Lombardy Prealps, northern Italy, are protected under Annex IV of European Community Directive 92/43/EEC. Seed germination dynamics of these species were studied with the aim of producing appropriate germination protocols for use in ex situ conservation. Seeds were collected from Monte Barro (922 m a.s.l.) and for Primula glaucescens from three additional sites in a more central part of its range (1217-2521 m a.s.l.). Physoplexis comosa germinated only in sterile in vitro conditions, in which germination percentages were extremely low without a supply of gibberellic acid (GA3; 1.7%) ; the optimum GA3 concentration was 100 mg l-1 (> 90% germination), with lower germination at higher doses of 250-500 mg l -1. Primula glaucescens germinated in both sterile and non-sterile conditions and, although total germination was not affected by GA3 concentration, doses of 10-500 mg l-1 acted equally to prompt earlier and more rapid germination. Primula glaucescens from Monte Barro was characterised by slower and poorer germination than from central sites.
|Titolo:||Seed germination and conservation of endangered species from the Italian Alps : Physoplexis comosa and Primula glaucescens|
PIERCE, SIMON (Penultimo)
|Parole Chiave:||Conservation; Endemic species; Germination; Gibberellic acid; Phytohormone|
|Settore Scientifico Disciplinare:||Settore BIO/03 - Botanica Ambientale e Applicata|
|Data di pubblicazione:||2004|
|Digital Object Identifier (DOI):||10.1016/j.biocon.2003.12.011|
|Appare nelle tipologie:||01 - Articolo su periodico|