OBJECTIVE. The interplay of genetic and nutritional regulation of the insulin-like growth factor-I axis in children is unclear. Therefore, potential gene–nutrient effects on serum levels of the IGF-I axis in a formula feeding trial were studied. DESIGN. European multicenter randomized clinical trial of 1090 term, formula-fed infants assigned to receive cow's milk-based infant and follow-on formulae with lower (LP: 1.25 and 1.6g/100mL) or higher (HP: 2.05 and 3.2g/100mL) protein contents for the first 12months of life; a comparison group of 588 breastfed infants (BF) was included. Eight single nucleotide polymorphisms (SNPs) of the IGF-1-(rs6214, rs1520220, rs978458, rs7136446, rs10735380, rs2195239, rs35767, and rs35766) and two of the IGFBP-3-(rs1496495, rs6670) gene were analyzed. Serum levels of total and free IGF-I, IGFBP-3 and the molar ratio IGF-1/IGFBP-3 at age 6months were regressed on determined SNPs and feeding groups in 501 infants. RESULTS. IGF-1-SNPs rs1520220, rs978458, and rs2195239 significantly increased total-IGF-I and molar-ratio IGF-I/IGFBP-3 by ~1.3ng/mL and ~1.3 per allele, respectively; compared to LP infants concentration and molar-ratio were increased in HP by ~1.3ng/mL and ~1.3 and decreased in BF infants by ~0.6ng/mL and ~0.6, respectively. IGFBP-3 was only affected by the BF group with ~450ng/mL lower levels than the LP group. No gene-feeding-group interaction was detected for any SNP, even without correction for multiple testing. CONCLUSIONS. Variants of the IGF-1-gene play an important role in regulating serum levels of the IGF-I axis but there is no gene-protein-interaction. The predominant nutritional regulation of IGF-I and IGFBP-3 gives further evidence that higher protein intake contributes to metabolic programming of growth.
Associations of IGF-1 gene variants and milk protein intake with IGF-I concentrations in infants at age 6 months : Results from a randomized clinical trial / P. Rzehak, V. Grote, E. Lattka, M. Weber, D. Gruszfeld, P. Socha, R. Closa-Monasterolo, J. Escribano, M. Giovannini, E. Verduci, P. Goyens, F. Martin, J.P. Langhendries, H. Demmelmair, N. Klopp, T. Illig, B. Koletzko. - In: GROWTH HORMONE & IGF RESEARCH. - ISSN 1096-6374. - 23:5(2013 Jun 24), pp. 149-158.
|Titolo:||Associations of IGF-1 gene variants and milk protein intake with IGF-I concentrations in infants at age 6 months : Results from a randomized clinical trial|
|Parole Chiave:||European childhood obesity trial study; IGF-1 gene; IGF-I concentration; IGFBP-3 variants; protein formula; randomized clinical trial|
|Settore Scientifico Disciplinare:||Settore MED/38 - Pediatria Generale e Specialistica|
Settore AGR/18 - Nutrizione e Alimentazione Animale
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
|Progetto:||Long-term effects of early nutrition on later health|
|Data di pubblicazione:||24-giu-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.ghir.2013.05.002|
|Appare nelle tipologie:||01 - Articolo su periodico|