Background/Aims: Graft re-infection invariably occurs after liver transplantation (OLT) for chronic hepatitis C and disease progression is unpredictable. We prospectively examined peripheral blood mononuclear cells (PBMC) subsets and natural killer (NK) cell receptors (NKRs) in patients with recurrent hepatitis C post-OLT. Methods: PBMC were obtained at baseline and at different time points after OLT. NKRs were identified using monoclonal antibodies by flow cytometry. Results: The proportions of NK, natural T (NT), total and γδ T cells were significantly reduced (p < 0.01) 7 days post-transplant, probably as a result of graft repopulation. NKG2D+ NK cells were significantly higher compared with healthy controls (p < 0.01), declined post-OLT and subsequently returned to baseline values. This, together with a progressive increase in the proportion of CD94/NKG2C+ NK cells over time (p ≤ 0.01), appeared to be related to hepatitis C recurrence. There was a statistically significant correlation between expression of the natural cytotoxicity receptors (NCRs) and ALT (p < 0.05), supporting the hypothesis that NK cells participate in the necroinflammatory process. Conclusions: The data are compatible with homing of immune cells to the liver allograft after surgery, most of which return to pre-OLT levels. HCV recurrence may cause variations in selected NKRs expression akin to other viral infections.
Prospective study of natural killer cell phenotype in recurrent hepatitis C virus infection following liver transplantation / S. Varchetta, B. Oliviero, M. Francesca Donato, F. Agnelli, C. Rigamonti, E. Paudice, E. Arosio, M. Berra, G. Rossi, C. Tinelli, F.F. Fagnoni, M. Colombo, D. Mavilio, M.U. Mondelli. - In: JOURNAL OF HEPATOLOGY. - ISSN 0168-8278. - 50:2(2009 Feb), pp. 314-322. ((Intervento presentato al 43. convegno Annual Meeting of the European-Association-for-the-Study-of-the-Liver tenutosi a Milano nel 2008.
|Titolo:||Prospective study of natural killer cell phenotype in recurrent hepatitis C virus infection following liver transplantation|
|Parole Chiave:||Innate immunity; Liver transplantation; Natural killer cells|
|Settore Scientifico Disciplinare:||Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio|
Settore MED/04 - Patologia Generale
Settore MED/17 - Malattie Infettive
Settore MED/12 - Gastroenterologia
|Data di pubblicazione:||feb-2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jhep.2008.10.018|
|Appare nelle tipologie:||01 - Articolo su periodico|