Spatiotemporal interactions play important roles in tissue development and function, especially in stem cell-seeded bioscaffolds. Cells interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation. In vitro cultures of different human progenitor cells, i.e. endothelial colony-forming cells (ECFCs) from a healthy control and a patient with Kaposi sarcoma (an angioproliferative disease) and human CD133+ muscle-derived stem cells (MSH 133+ cells), were seeded onto polyglycolic acid–polylactic acid scaffolds. 3D images were obtained by X-ray phase-contrast microtomography (micro-CT) and processed with the Modified Bronnikov Algorithm. The method enabled high spatial resolution detection of the 3D structural organization of cells on the bioscaffold and evaluation of the way and rate at which cells modified the construct at different time points from seeding. The different cell types displayed significant differences in proliferation rate. In conclusion, X-ray synchrotron radiation phase-contrast micro-CT analysis proved to be a useful and sensitive tool to investigate the spatiotemporal pattern of progenitor cell organization on a bioscaffold.

Polyglycolic acid–polylactic acid scaffold response to different progenitor cell in vitro cultures : a demonstrative and comparative x-ray synchrotron radiation phase-contrast microtomography study / A. Giuliani, F. Moroncini, S. Mazzoni, M. Belicchi, C. Villa, S. Erratico, E. Colombo, F. Calcaterra, L. Brambilla, Y. Torrente, G. Albertini, S.A.M. Della Bella. - In: TISSUE ENGINEERING, PART C: METHODS. - ISSN 1937-3384. - 20:4(2014), pp. 308-316. [10.1089/ten.TEC.2013.0213]

Polyglycolic acid–polylactic acid scaffold response to different progenitor cell in vitro cultures : a demonstrative and comparative x-ray synchrotron radiation phase-contrast microtomography study

C. Villa;S. Erratico;E. Colombo;F. Calcaterra;Y. Torrente;S.A.M. Della Bella
2014

Abstract

Spatiotemporal interactions play important roles in tissue development and function, especially in stem cell-seeded bioscaffolds. Cells interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation. In vitro cultures of different human progenitor cells, i.e. endothelial colony-forming cells (ECFCs) from a healthy control and a patient with Kaposi sarcoma (an angioproliferative disease) and human CD133+ muscle-derived stem cells (MSH 133+ cells), were seeded onto polyglycolic acid–polylactic acid scaffolds. 3D images were obtained by X-ray phase-contrast microtomography (micro-CT) and processed with the Modified Bronnikov Algorithm. The method enabled high spatial resolution detection of the 3D structural organization of cells on the bioscaffold and evaluation of the way and rate at which cells modified the construct at different time points from seeding. The different cell types displayed significant differences in proliferation rate. In conclusion, X-ray synchrotron radiation phase-contrast micro-CT analysis proved to be a useful and sensitive tool to investigate the spatiotemporal pattern of progenitor cell organization on a bioscaffold.
Stem-cells; computed-tomography; kaposis-sarcoma; bone; bladder; biomaterials; regeneration; cartilage; herpesvirus; adhesion
Settore MED/04 - Patologia Generale
Settore MED/26 - Neurologia
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore FIS/01 - Fisica Sperimentale
2014
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/223084
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