Mutations have been identified in a non-canonical Wnt signalling cascade (the planar cell polarity pathway) in several mouse genetic models of severe neural tube defects. In each of these models, neurulation fails to be initiated at the 3-4 somite stage, leading to an almost entirely open neural tube (termed craniorachischisis). Studies in whole embryo culture have identified a defect in the morphogenetic process of convergent extension during gastrulation, preceding the onset of neural tube closure. The principal defect is a failure of midline extension, both in the neural plate and axial mesoderm. This leads to an abnormally wide neural plate in which the elevating neural folds are too far apart to achieve closure. In this chapter, we provide details of several experimental methods that can be used to evaluate convergent extension in cultured mouse embryos. We describe analytical methods that can reveal the abnormalities that characterise neurulation-stage embryos with defective planar cell polarity signalling, in particular the loop-tail (Lp; Vangl2) mutant.

Convergent extension analysis in mouse whole embryo culture / S.E. Pryor, V. Massa, D. Savery, N.D. Greene, A.J. Copp (METHODS IN MOLECULAR BIOLOGY). - In: Planar cell polarity : methods and protocols / [a cura di] K. Turksen. - [s.l] : Humana Press, 2012. - ISBN 9781617795091. - pp. 133-146 [10.1007/978-1-61779-510-7_11]

Convergent extension analysis in mouse whole embryo culture

V. Massa
Secondo
;
2012

Abstract

Mutations have been identified in a non-canonical Wnt signalling cascade (the planar cell polarity pathway) in several mouse genetic models of severe neural tube defects. In each of these models, neurulation fails to be initiated at the 3-4 somite stage, leading to an almost entirely open neural tube (termed craniorachischisis). Studies in whole embryo culture have identified a defect in the morphogenetic process of convergent extension during gastrulation, preceding the onset of neural tube closure. The principal defect is a failure of midline extension, both in the neural plate and axial mesoderm. This leads to an abnormally wide neural plate in which the elevating neural folds are too far apart to achieve closure. In this chapter, we provide details of several experimental methods that can be used to evaluate convergent extension in cultured mouse embryos. We describe analytical methods that can reveal the abnormalities that characterise neurulation-stage embryos with defective planar cell polarity signalling, in particular the loop-tail (Lp; Vangl2) mutant.
Convergent extension; Craniorachischisis; DiI labelling; Electroporation; Loop-tail; Mouse; Neural tube defects; Neurulation; Vangl2; Whole embryo culture
Settore BIO/13 - Biologia Applicata
2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/223041
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