We investigated 42 plasmas prepared at different centrifugation speeds with three activated protein C (APC) resistance methods. The APC ratio for fresh platelet-poor plasma declined significantly after freezing and thawing. This effect was more evident with the original method (average reduction 11.3%) than with either the home-made (3.8%) or the modified method (3.2%). No significant decrease in the APC ratio was observed after freezing and thawing of platelet-free plasmas from the same patients. When frozen platelet-poor plasma was centrifuged at high speed after thawing and before testing, there was no significant decrease in the APC ratio, in comparison with fresh platelet-poor plasma using the home-made and modified methods.

Standardization of activated protein C resistance testing : effect of residual platelets in frozen plasmas assessed with commercial and home made methods / A. Tripodi, C. Valsecchi, V. Chantarangkul, T. Battaglioli, P.M. Mannucci. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - 120:5(2003), pp. 825-828.

Standardization of activated protein C resistance testing : effect of residual platelets in frozen plasmas assessed with commercial and home made methods

A. Tripodi;P.M. Mannucci
2003

Abstract

We investigated 42 plasmas prepared at different centrifugation speeds with three activated protein C (APC) resistance methods. The APC ratio for fresh platelet-poor plasma declined significantly after freezing and thawing. This effect was more evident with the original method (average reduction 11.3%) than with either the home-made (3.8%) or the modified method (3.2%). No significant decrease in the APC ratio was observed after freezing and thawing of platelet-free plasmas from the same patients. When frozen platelet-poor plasma was centrifuged at high speed after thawing and before testing, there was no significant decrease in the APC ratio, in comparison with fresh platelet-poor plasma using the home-made and modified methods.
standardization; activated protein C (APC) resistance; platelets; thrombophilia
Settore MED/15 - Malattie del Sangue
Settore MED/09 - Medicina Interna
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/22258
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