DNA double-strand breaks (DSBs) are the most cytotoxic form of DNA damage, since they can lead to genome instability and chromosome rearrangements, which are hallmarks of cancer cells. To face this kind of lesion, eukaryotic cells developed two alternative repair pathways, homologous recombination (HR) and non-homologous end joining (NHEJ). Repair pathway choice is influenced by the cell cycle phase and depends upon the 5′-3′ nucleolytic processing of the break ends, since the generation of ssDNA tails strongly stimulates HR and inhibits NHEJ. A large amount of work has elucidated the key components of the DSBs repair machinery and how this crucial process is finely regulated. The emerging view suggests that besides endo/exonucleases and helicases activities required for end resection, molecular barrier factors are specifically loaded in the proximity of the break, where they physically or functionally limit DNA degradation, preventing excessive accumulation of ssDNA, which could be threatening for cell survival.

To trim or not to trim: progression and control of DSB end resection / M. Granata, D. Panigada, E. Galati, F. Lazzaro, A. Pellicioli, P. Plevani, M. Muzi-Falconi. - In: CELL CYCLE. - ISSN 1538-4101. - 12:12(2013), pp. 1848-1860. [10.4161/cc.25042]

To trim or not to trim: progression and control of DSB end resection

M. Granata
Primo
;
D. Panigada
Secondo
;
E. Galati;F. Lazzaro;A. Pellicioli;P. Plevani
Penultimo
;
M. Muzi-Falconi
Ultimo
2013

Abstract

DNA double-strand breaks (DSBs) are the most cytotoxic form of DNA damage, since they can lead to genome instability and chromosome rearrangements, which are hallmarks of cancer cells. To face this kind of lesion, eukaryotic cells developed two alternative repair pathways, homologous recombination (HR) and non-homologous end joining (NHEJ). Repair pathway choice is influenced by the cell cycle phase and depends upon the 5′-3′ nucleolytic processing of the break ends, since the generation of ssDNA tails strongly stimulates HR and inhibits NHEJ. A large amount of work has elucidated the key components of the DSBs repair machinery and how this crucial process is finely regulated. The emerging view suggests that besides endo/exonucleases and helicases activities required for end resection, molecular barrier factors are specifically loaded in the proximity of the break, where they physically or functionally limit DNA degradation, preventing excessive accumulation of ssDNA, which could be threatening for cell survival.
DNA repair ; chromatin ; double-strand breaks ; homologous recombination ; non-homologous end joining ; nucleases
Settore BIO/11 - Biologia Molecolare
Settore BIO/18 - Genetica
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/221652
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