The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a beta-cyclodextrin-poly (4-acryloylmorpholine) monoconjugate (beta-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be described in this chapter. Fluorescent labeled nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.
Enhanced antiviral activity of acyclovir loaded into nanoparticles / R. Cavalli, M. Donalisio, A. Bisazza, A. Civra, E. Ranucci, P. Ferruti, D. Lembo - In: Nanomedicine: infectious diseases, immunotherapy, diagnostics, antifibrotics, toxicology and gene medicine / [a cura di] N. Düzgüneş. - San Diego : Academic Press, 2012. - ISBN 9780123918581. - pp. 1-19 [10.1016/B978-0-12-391858-1.00001-0]
Enhanced antiviral activity of acyclovir loaded into nanoparticles
E. Ranucci;P. FerrutiPenultimo
;
2012
Abstract
The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a beta-cyclodextrin-poly (4-acryloylmorpholine) monoconjugate (beta-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be described in this chapter. Fluorescent labeled nanoparticles are prepared using the same method for cellular trafficking studies. The biocompatibility assays necessary to obtain safe nanoparticles are reported. Section 4 of this chapter describes the assessment of the antiviral activity of the acyclovir-loaded nanoparticles.
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