Pseudomonas aeruginosa is an important opportunistic pathogen in immune-compromised and cystic fibrosis patients. We have performed a comparative small RNA (sRNA) profiling via deep-sequencing of P. aeruginosa PAO1 and PA14 strains (1) which share the same host range but differ in pathogenicity. We now aim at identifying infection-relevant sRNA-based regulatory systems. To this end, we are focusing on a short list of 8 validated sRNAs which showed, at preliminary screenings, potential virulence hallmarks such as: specific expression in PA14 or PAO1; responsiveness to infection-relevant host stimuli (e.g. oxygen availability, temperature shift) or invasion signals (e.g. quorum sensing). Our approach is to generate sRNA deletion mutants and test them for i) canonical virulence phenotype(s); ii) ability to invade human respiratory epithelial cells and stimulate an immune response; iii) airways infection in both acute and chronic murine models. The above tests are accompanied by in silico, genetic and transcriptomics-based screenings for the genome-wide search of sRNAs target genes. 1. Ferrara S et al. (2012). PLoS One 7: e36553.

Evaluation of the infection-relevant role of small RNA-based regulatory systems in the opportunistic pathogen Pseudomonas aeruginosa / S. Carloni, F. Delvillani, J. Georg, W.R. Hess, F. Briani, G. Bertoni, S. Ferrara. ((Intervento presentato al 12. convegno FISV tenutosi a Roma nel 2012.

Evaluation of the infection-relevant role of small RNA-based regulatory systems in the opportunistic pathogen Pseudomonas aeruginosa

S. Carloni
Primo
;
F. Delvillani
Secondo
;
F. Briani;G. Bertoni
Penultimo
;
S. Ferrara
Ultimo
2012

Abstract

Pseudomonas aeruginosa is an important opportunistic pathogen in immune-compromised and cystic fibrosis patients. We have performed a comparative small RNA (sRNA) profiling via deep-sequencing of P. aeruginosa PAO1 and PA14 strains (1) which share the same host range but differ in pathogenicity. We now aim at identifying infection-relevant sRNA-based regulatory systems. To this end, we are focusing on a short list of 8 validated sRNAs which showed, at preliminary screenings, potential virulence hallmarks such as: specific expression in PA14 or PAO1; responsiveness to infection-relevant host stimuli (e.g. oxygen availability, temperature shift) or invasion signals (e.g. quorum sensing). Our approach is to generate sRNA deletion mutants and test them for i) canonical virulence phenotype(s); ii) ability to invade human respiratory epithelial cells and stimulate an immune response; iii) airways infection in both acute and chronic murine models. The above tests are accompanied by in silico, genetic and transcriptomics-based screenings for the genome-wide search of sRNAs target genes. 1. Ferrara S et al. (2012). PLoS One 7: e36553.
set-2012
Settore BIO/18 - Genetica
Settore BIO/19 - Microbiologia Generale
Settore BIO/11 - Biologia Molecolare
Evaluation of the infection-relevant role of small RNA-based regulatory systems in the opportunistic pathogen Pseudomonas aeruginosa / S. Carloni, F. Delvillani, J. Georg, W.R. Hess, F. Briani, G. Bertoni, S. Ferrara. ((Intervento presentato al 12. convegno FISV tenutosi a Roma nel 2012.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/218246
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