Aim: Pregnancy is characterized by left ventricular hypertrophy that is potentially accounted for by cardiomyocyte proliferation, although no such evidence is currently available. This study investigates if the left ventricular mass (LVM) increase during pregnancy implies cell hyperplasia. Materials & methods: In nonpregnant and late-pregnant rats, cardiac function and LVM were evaluated by MRI, and cardiomyocyte dimensions and proliferations were assessed quantitatively by morphometric analysis and immunohistochemistry using oncological markers (Ki67 and MCM2). Results: In late-pregnant rats, LVM and cardiomyocyte area were greater. No mitotic figures were found nor was there any significant difference between groups in Ki67 expression. MCM2 expression was related to LVM. Conclusion: During pregnancy, rat cardiomyocytes undergo hypertrophy but not hyperplasia; the expression of MCM2, related to LVM, suggests it could be a marker of protein synthesis. The application of oncological markers to physiological contexts may provide insight into their role within the cell cycle

Immunohistochemical expression of oncological proliferation markers in the hearts of rats during normal pregnancy / M.M. Ciulla, G. Acquistapace, G.L. Perrucci, P. Nicolini, L. Toffetti, P. Braidotti, S. Ferrero, I. Zucca, D. Aquino, G. Busca, F. Magrini. - In: BIOMARKERS IN MEDICINE. - ISSN 1752-0363. - 7:1(2013 Feb), pp. 119-129. [10.2217/bmm.12.94]

Immunohistochemical expression of oncological proliferation markers in the hearts of rats during normal pregnancy

M.M. Ciulla;G.L. Perrucci;P. Nicolini;P. Braidotti;S. Ferrero;G. Busca;F. Magrini
2013

Abstract

Aim: Pregnancy is characterized by left ventricular hypertrophy that is potentially accounted for by cardiomyocyte proliferation, although no such evidence is currently available. This study investigates if the left ventricular mass (LVM) increase during pregnancy implies cell hyperplasia. Materials & methods: In nonpregnant and late-pregnant rats, cardiac function and LVM were evaluated by MRI, and cardiomyocyte dimensions and proliferations were assessed quantitatively by morphometric analysis and immunohistochemistry using oncological markers (Ki67 and MCM2). Results: In late-pregnant rats, LVM and cardiomyocyte area were greater. No mitotic figures were found nor was there any significant difference between groups in Ki67 expression. MCM2 expression was related to LVM. Conclusion: During pregnancy, rat cardiomyocytes undergo hypertrophy but not hyperplasia; the expression of MCM2, related to LVM, suggests it could be a marker of protein synthesis. The application of oncological markers to physiological contexts may provide insight into their role within the cell cycle
Settore MED/11 - Malattie dell'Apparato Cardiovascolare
Settore MED/04 - Patologia Generale
Settore MED/08 - Anatomia Patologica
Settore MED/40 - Ginecologia e Ostetricia
Settore MED/36 - Diagnostica per Immagini e Radioterapia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/216025
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