This PhD thesis is dedicated to the preparation, characterisation and biological application of gold nanoconjugates. Gold nanoparticles are prepared by various modern preparation methods, and subsequently characterised by spectroscopic (UV-vis, ATR-FTIR, NMR), microscopic (TEM) techniques. The stability of the conjugates is evaluated both by using Zeta-potential studies and UV-vis. The nanoparticles are used in cellular uptake experiments using human glioblastoma cancer cells, and are found to possess a low cytotoxicity. The nanoparticles are found taken up by the cells and distributed in different cellular compartments.
GOLD NANOCONJUGATES: PREPARATION, CHARACTERISATION AND BIOLOGICAL APPLICATIONS / S. Avvakumova ; tutor: F. Porta, G. Scari ; coordinatore: D. Robertò. DIPARTIMENTO DI SCIENZE BIOMEDICHE E CLINICHE "L. SACCO", 2013 Jan 11. 25. ciclo, Anno Accademico 2012. [10.13130/avvakumova-svetlana_phd2013-01-11].
GOLD NANOCONJUGATES: PREPARATION, CHARACTERISATION AND BIOLOGICAL APPLICATIONS
S. Avvakumova
2013
Abstract
This PhD thesis is dedicated to the preparation, characterisation and biological application of gold nanoconjugates. Gold nanoparticles are prepared by various modern preparation methods, and subsequently characterised by spectroscopic (UV-vis, ATR-FTIR, NMR), microscopic (TEM) techniques. The stability of the conjugates is evaluated both by using Zeta-potential studies and UV-vis. The nanoparticles are used in cellular uptake experiments using human glioblastoma cancer cells, and are found to possess a low cytotoxicity. The nanoparticles are found taken up by the cells and distributed in different cellular compartments.
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