The psae1-1 mutant of Arabidopsis was identified on the basis of a decrease in the effective quantum yield of photosystem II, among a collection of plants subjected to transposon tagging with the Enhancer element. The steady-state redox level and the rate of re-oxidation of P700 are significantly altered in psae1-1 mutants. The responsible mutation was localised to psaE1, one of two Arabidopsis genes that encode subunit E of photosystem I. An additional mutant allele, psae1-2, was identified by reverse genetics. In wild-type plants, the psaE1 transcript is expressed at a higher level than psaE2 mRNA. In the mutants, however, the E1 transcript was barely detectable, and was expressed only in small groups of wild-type cells resulting from somatic reversions. As a consequence, the amount of PsaE protein present in the mutant is significantly reduced. Concomitantly, the levels of other stromal photosystem I subunits (PsaC and PsaD) are also affected. Mutant plants showed a marked increase in light sensitivity and photoinhibition. Additional effects of the psae1 mutation include light green pigmentation, an increase in chlorophyll fluorescence and a decrease of approximately 50% in growth rate under greenhouse conditions.
Disruption of the Arabidopsis photosystem I gene PsaE1 affects photosynthesis and impairs growth / C. Varotto, P. Pesaresi, J. Meurer, R. Oelmueller, S. Steiner-Lange, F. Salamini, D. Leister. - In: PLANT JOURNAL. - ISSN 0960-7412. - 22:2(2000), pp. 115-124.
|Titolo:||Disruption of the Arabidopsis photosystem I gene PsaE1 affects photosynthesis and impairs growth|
PESARESI, PAOLO (Secondo)
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
|Data di pubblicazione:||2000|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1046/j.1365-313x.2000.00717.x|
|Appare nelle tipologie:||01 - Articolo su periodico|