Acetyl-LDL concentration-dependently increase the PAI-1 antigen levels in conditioned medium of human umbilical vein endothelial cells (HUVEC) and this effect is due to "de-novo" protein synthesis. In this study we have evaluated whether the effect of acetyl-LDL on PAI-1 biosynthesis by HUVEC was mediated by the interaction of the lipoprotein with the "scavenger" receptor. Fucoidan and dextran sulphate but not condroitin sulfate concentration-dependently prevented the effect of acetyl-LDL (25 µg/ml) on PAI-1 antigen release by HUVEC. Since the interaction of acetyl-LDL with the scavenger receptor is followed by intracellular lipid accumulation, experiments were carried out with free exogenous non-lipoprotein cholesterol and triglycerides rich particles, isolated from Intralipid 20% . PAI-1 levels were not affected by either cholesterol or triglyceride enrichment of cells. 1t is concluded that the activation of the scavenger receptor by acetyl-LDL in endothelial cells is, at least partially, involved in the induction of PAI-1 biosynthesis.
REGULATION OF PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 BY ACETYL-LDL IN HUMAN UMBIUCAL VEIN ENDOTIIELIAL CELLS / M. Camera, C. Banfi, L. Mussoni, L. Prati, D. Baldassarre, F. Bernini, E. Tremoli. ((Intervento presentato al 12. convegno INTERNATIONAL SYMPOSIUM OF DRUG AFFECTING LIPID METABOLISM tenutosi a Houston (Texas) nel 1995.
REGULATION OF PLASMINOGEN ACTIVATOR INHIBITOR TYPE-1 BY ACETYL-LDL IN HUMAN UMBIUCAL VEIN ENDOTIIELIAL CELLS
M. CameraPrimo
;C. BanfiSecondo
;L. Mussoni;D. Baldassarre;E. TremoliUltimo
1995
Abstract
Acetyl-LDL concentration-dependently increase the PAI-1 antigen levels in conditioned medium of human umbilical vein endothelial cells (HUVEC) and this effect is due to "de-novo" protein synthesis. In this study we have evaluated whether the effect of acetyl-LDL on PAI-1 biosynthesis by HUVEC was mediated by the interaction of the lipoprotein with the "scavenger" receptor. Fucoidan and dextran sulphate but not condroitin sulfate concentration-dependently prevented the effect of acetyl-LDL (25 µg/ml) on PAI-1 antigen release by HUVEC. Since the interaction of acetyl-LDL with the scavenger receptor is followed by intracellular lipid accumulation, experiments were carried out with free exogenous non-lipoprotein cholesterol and triglycerides rich particles, isolated from Intralipid 20% . PAI-1 levels were not affected by either cholesterol or triglyceride enrichment of cells. 1t is concluded that the activation of the scavenger receptor by acetyl-LDL in endothelial cells is, at least partially, involved in the induction of PAI-1 biosynthesis.Pubblicazioni consigliate
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