Plastocyanin is a soluble copper-containing protein present in the thylakoid lumen, which transfers electrons to photosystem I. In the chloroplast of the flowering plant Arabidopsis thaliana, a cytochrome c6-like protein is present, which was recently suggested to function as an alternative electron carrier to plastocyanin. We show that Arabidopsis plants mutated in both of the two plastocyanin-coding genes and with a functional cytochrome c6 cannot grow photoautotrophically because of a complete block in light-driven electron transport. Even increased dosage of the gene encoding the cytochrome c6-like protein cannot complement the double mutant phenotype. This demonstrates that in Arabidopsis only plastocyanin can donate electrons to photosystem I in vivo.
|Titolo:||Plastocyanin Is Indispensable for Photosynthetic Electron Flow in Arabidopsis thaliana|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
Settore BIO/18 - Genetica
|Data di pubblicazione:||15-ago-2003|
|Digital Object Identifier (DOI):||10.1074/jbc.M302876200|
|Appare nelle tipologie:||01 - Articolo su periodico|