A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
A rapid method for monitoring DNA labelling reactions with haptens / A. Corti, P. Tripputi, G. Cassani - In: International Symposium on Biotechnology of Plasmaprotein : Haemostasis,Thombosis and Iron Protein[s.l] : Società italiana per lo studio dell'emostasi e della trombosi, 1990. - pp. 81-86 (( convegno International Symposium on Biotechnology of Plasmaprotein : Haemostasis,Thombosis and Iron Protein tenutosi a Firenze nel 1990 [10.1016/0022-1759(90)90114-B].
A rapid method for monitoring DNA labelling reactions with haptens
P. TripputiSecondo
;
1990
Abstract
A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
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