A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
Rapid detection of hapten-labeled DNA by flow-through dot immunobinding assay / A. Corti, P. Tripputi, G. Cassani - In: Biotechnology of Plasma Proteins: Haemostasis, Thrombosis and Iron Proteins International Symposium, Florence, April 1990 / [a cura di] A. Albertini; C.L. Lenfant; P.M. Mannucci; J.J. Sixma. - [s.l] : Società italiana per lo studio dell'emostasi e della trombosi; Karger, 1991. - ISBN 978-3-318-03528-5. (( convegno International Symposium on Biotechnology of Plasmaprotein : Haemostasis,Thombosis and Iron Protein tenutosi a Firenze nel 1990.
Rapid detection of hapten-labeled DNA by flow-through dot immunobinding assay
P. TripputiSecondo
;
1991
Abstract
A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
